MagSi-NGSPREP allows selective binding of DNA with a size cut-off between 100bp and 1kb with particular reagent volume: sample volume ratios. By increasing the volume of MagSi-NGSPREP, the effectiveness of binding smaller sized fragments increases. This permits the user to keep or discard undesired fragment sizes selectively. Based on which protocol is used, total preparation period using MagSi-NGSPREP is 20-30 moments and the hands-on time necessary for the whole method is reduced to a minimum.He by no means completed the descent, however, and he appeared to be experiencing confusion – – a common indicator of hypoxia . At one stage he became worried about his plane’s reserve fuel source and was cleared to property at ‘Taylor,’ but he was struggling to comply. With the pilot nonresponsive, the aircraft left Atlanta airspace and flew toward Cuba directly. It crossed Cuba and then crashed into the ocean soon after, presumably due to gas starvation of the engine . Hear the edited sound here: Transcript of N900KN conversations with atmosphere traffic control before the pilot lost consciousnessPilot: TBM 900KN trip level 280 ATC: November 900KN Atlanta.